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Circular dichroism spectra of NK4-M2GlyR p22 S22W and the NK4-M2GlyR p27 WT sequences. (A) NK4-M2GlyR WT (▵) in 40% TFE; (○) in H2O; and (▪) in 10 mM SDS. (B) NK4-M2GlyR S22W (▵) in 40% TFE; (○) in H2O; and (▪) in 10 mM SDS. Peptide concentrations were 50 μM. Spectra were recorded at ambient temperature. Spectra are averages of eight scans after subtraction of the reference spectra of the media.
