Summary histogram of the mean relative outward rectification values for BIB mutant channels. Compiled data for the relative outward rectification, measured as the ratio of current amplitudes at +50 and −70 mV, in salines with Mg2+ (solid), Ca2+ (open), or Ba2+ (cross-hatched). Statistically significant differences are shown as ***p < 0.001, **p < 0.01, and *p < 0.05. n-values are shown in parentheses; see text for summary data.