Figure 3. GACC suppresses proliferation-associated and β-catenin-associated transcripts and reduces dysplastic features in tert transgenic zebrafish. (A–F) Quantitative polymerase chain reaction (qPCR) analysis of liver tissue from Tg(fabp10a:tert) zebrafish treated by immersion with vehicle (0.1% DMSO) or GACC (50 µM) for 15 days. Relative mRNA expression of cell proliferation markers ((A) ccne1, (B) cdk1, (C) cdk2) and β-catenin-associated target genes ((D) myca, (E) mycb, (F) ccnd1) is shown, normalized to β-actin and expressed relative to vehicle. Data are mean ± SEM; n > 3 biological replicates (each replicate = pooled livers from 10 fish). (G–J) Liver histopathological in the same treatment groups. (G,H) Quantification of histologic endpoints including (G) mitotic figures, (H) trinucleated cells, and (I) karyomegalic hepatocytes (scoring method and fields per fish as described in Methods). Data are mean ± SEM; n = 10 fish per group; analysis performed by a blinded assessor. (J) Representative H&E-stained sections showing hepatic architecture and cellular atypia. Scale bar: 20 µm. Statistical tests: one-way ANOVA with Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.