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. 2004 Apr;86(4):2058–2077. doi: 10.1016/S0006-3495(04)74267-8

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Examples of fits of the computed double-cell acceptor-box/donor-box ratio (Eq. 22) as a function of time (solid curves) to corresponding fluorescence intensity data (points). Calculations are based on the coarse mesh. The key adjustable parameter is the (dimensionless) intercellular membrane permeability , marked for each curve. BG accounts for background signal resulting from autofluorescence and/or light refraction. (A) Alexa 350 in Cx37/Cx43 heterotypic channels. (B) Alexa 488 in Cx26/Cx26 homotypic channels. The execution time required to generate each calculated curve is ∼7 min for A and 12 min for B (see computer specifications given in Fig. 5 legend).

Inline graphic — Examples of fits of the computed double-cell acceptor-box/donor-box ratio (Eq. 22) as a function of time (solid curves) to corresponding fluorescence intensity data (points). Calculations are based on the coarse mesh. The key adjustable parameter is the (dimensionless) intercellular membrane permeability , marked for each curve. BG accounts for background signal resulting from autofluorescence and/or light refraction. (A) Alexa 350 in Cx37/Cx43 heterotypic channels. (B) Alexa 488 in Cx26/Cx26 homotypic channels. The execution time required to generate each calculated curve is ∼7 min for A and 12 min for B (see computer specifications given in Fig. 5 legend).