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Burst times are substantially increased by all 171 substitutions that express. The dramatic disruption of gating suggests that T171 is required for wild-type gate closure and that no other amino acid residue at the position substitutes well for threonine. The dashed line indicates the value of the wild-type mean burst time in each of the duration histograms. For mean burst time values of each channel, n ≥ 4, with mutant values significantly different from wild-type values, P < 0.001.
