FIGURE 3.

Closed time distributions of 171 substitutions show nearly complete loss of long-lived interburst closed times, with little or no effect on the short-lived intraburst closed times. The dashed line indicates the value of the wild-type mean long-lived closed time of 15.7 ms in each of the duration histograms. Note that the relatively few long-lived closed events are consistent with but cannot differentiate between the 171 substitutions either greatly slowing gate closure or dramatically disrupting its shut state stability.