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. 2004 Apr;86(4):2145–2155. doi: 10.1016/S0006-3495(04)74274-5

FIGURE 4.

FIGURE 4

A sustained positive holding potential deactivates the strongly rectifying C13 in a manner that depends on external K (indicated). The solid bar indicates a holding potential maintained at 100 mV more positive than the resting potential. There was no outward current during this period. The average resting potentials (Vm) were approximately Nernstian: −82.6 ± 1 mV for 3 mM external K, −55.1 ± 1.4 mV for 10 mM external K, −35.2 ± 1.1 mV for 25 mM external K, and −4.1 ± 1 mV for 100 mM external K. During the period of two-electrode voltage clamp with intact oocytes, internal pH was maintained at 7.04 by external acetate buffer at pHo = 7.8. Inward conductance was measured periodically using 20-ms voltage steps to generate momentary inward current.