FIGURE 8.
Effect of external spermine + spermidine on inward conductance of the chimera/mutant C13-K61M. Oocytes were initially equilibrated in 1 mM external K until a steady state was achieved. At this point, spermidine and spermine were added to the external solution. The oocyte remained unclamped throughout the experiment and inward conductance was periodically measured. A typical I-V curve for this mutant in low external K is shown in the inset. There was no significant difference after addition of external polyamines. Internal pH was maintained at 7.04 by external acetate buffer at pHo = 7.8.