FIGURE 3.
Actin labeled with FITC-phalloidin (green) and myosin labeled with essential light chain coupled to IATR (red) in the same muscle fiber. (A) Muscle viewed through fluorescein filter (band-pass 515 < λ < 565 nm). Only the I-bands, containing fluorescent actin, are visible. (B) The same fiber viewed through rhodamine filter (long-pass λ > 590 nm). Only the A-bands, containing fluorescent myosin, are visible. (C) Composite of A and B. Sarcomere length = 2.65 μm.