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(A) Fiber labeled with Alexa-ATP. The I-bands are fluorescent. Fiber viewed through Cy5 filter (long-pass λ > 675 nm). Bar = 10 μm. (B) Excitation anisotropy of Alexa-ATP. 1 μM of free Alexa-ATP in Ca-rigor, ▾; 2 μM F-actin + 1 μM Alexa-ATP in Ca-rigor, •; and 2 μM F-actin + 1 μM Alexa-ATP in 90% glycerol, ▪. Emission wavelength = 670 nm, 0°C.
