TABLE 1.

Composition of solutions

Solution	MgCl2	CaCl2	EGTA	EDTA	ATP	KCl
G-buffer		0.1			0.2
EGTA-rigor	4		2			50
EDTA-rigor				2		50
Ca2+-rigor	4	0.1				50
Relaxing	4		2		5	50
Glycerinating	2		5		5	50

All solutions contained 10 mM Tris-HCl buffer, pH 7.5. Ca²⁺-rigor solution contained 10 mM glutathione. Concentrations in mM.