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. 2004 Apr;86(4):2318–2328. doi: 10.1016/S0006-3495(04)74289-7

FIGURE 4.

FIGURE 4

The two-photon microscopy images of a single rabbit psoas muscle fiber loaded with 20 μM deac-edaADP before (A) and after (B) photolysis of 2 mM caged ADP. The two-photon excitation (760 nm) of the deac-edaADP fluorescence imaged at 470–490 nm. A striation pattern with an ∼2-μm repeat was observed before photolysis. The ridge along the longitudinal axis of the fiber in B reflects a fold in the surface. The fluorescence after photolytic release of ADP indicates displacement of the fluorophore from myosin to give a homogeneous distribution.