TABLE 2.
“Denaturant” concentrations at the midtransition point (Cm), and slope (m), intercept (ΔG°H2O), and squared correlation coefficient (r2) of the free energy versus “denaturant” concentration (Eq. 4), for the conformational transitions of β-lactoglobulin induced by DTAC and GnHCl assuming a two-state equilibrium
| Transitions induced by DTAC
| ||||
|---|---|---|---|---|
| Spectroscopic signal | Cm (mM) | m (kcal mol−1 M−1) | ΔG°H2O (kcal mol−1) | r2 |
| [θ]222 | 10.6 | −66 | 0.7 | 0.966 |
| [θ]293 | 3.2 | −530 | 1.7 | 0.803 |
| IF max | 5.8 | −240 | 1.4 | 0.941 |
| Transitions induced by GnHCl
| ||||
| Spectroscopic signal | Cm (M) | m (kcal mol−1 M−1) | ΔG°H2O (kcal mol−1) | r2 |
| [θ]222 | 2.8 | −1.2 | 3.2 | 0.975 |
| [θ]293 | 2.7 | −4.2 | 11.2 | 0.969 |
| IF max | 2.9 | −2.1 | 6.0 | 0.985 |