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. 2004 Apr;86(4):2403–2413. doi: 10.1016/S0006-3495(04)74297-6

FIGURE 4.

FIGURE 4

SDS-PAGE gels of spc-SH3 and lysozyme samples submitted to cross-linking with glutaraldehyde at two different temperatures and several protein concentrations. (A) 20°C; lanes 1–5: lysozyme in 20 mM glycine, pH 2.0; lanes 6–10: spc-SH3 in 20 mM glycine, pH 3.0. Lanes 1 and 6 correspond to samples not cross-linked. Lysozyme concentrations: lane 2, 1.92 mM; lane 3, 0.38 mM; lane 4, 0.07 mM; and lane 5, 0.014 mM. spc-SH3 concentrations: lane 7, 3.4 mM; lane 8, 0.69 mM; lane 9, 0.15 mM; and lane 10, 0.042 mM. (B) 50°C, lanes 1–5: lysozyme in 20 mM glycine, pH 2.0; lanes 6–10: SH3 in 20 mM glycine, pH 3.0. Lanes 1 and 6 correspond to samples not cross-linked. Lysozyme concentrations: lane 2, 0.021 mM; lane 3, 0.063 mM; lane 4, 0.31 mM; and lane 5, 1.8 mM. SH3 concentrations: lane 7, 0.042 mM; lane 8, 0.15 mM; lane 9, 0.53 mM; and lane 10, 2.6 mM. Lane M in both A and B gels corresponds to molecular weight markers.