Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Apr;86(4):2530–2537. doi: 10.1016/S0006-3495(04)74308-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, Biophysical Society

PMC Copyright notice

FRET changes a function of [NaCl] reflect changes in average distance. (A) Bulk solution fluorescence emission spectra of 20 nM dT23 for several [NaCl]. Anticorrelated behavior is seen between the donor and acceptor intensities as the [NaCl] is changed. (B) The total intensity, , does not vary much with [NaCl]; however their ratio, , increases with [NaCl] more drastically indicating changes in FRET. (C) Quantum yield (Φ^D) of Cy3-dT23 and absorbance of Cy5-dT23 (170 nM) at various [NaCl]. (D) Anisotropy of Cy3-dT23 and Cy5-dT23 at various [NaCl].

Inline graphic — FRET changes a function of [NaCl] reflect changes in average distance. (A) Bulk solution fluorescence emission spectra of 20 nM dT23 for several [NaCl]. Anticorrelated behavior is seen between the donor and acceptor intensities as the [NaCl] is changed. (B) The total intensity, , does not vary much with [NaCl]; however their ratio, , increases with [NaCl] more drastically indicating changes in FRET. (C) Quantum yield (Φ^D) of Cy3-dT23 and absorbance of Cy5-dT23 (170 nM) at various [NaCl]. (D) Anisotropy of Cy3-dT23 and Cy5-dT23 at various [NaCl].