TABLE 1.

FRET measurements calculated by I_A/(I_A + I_D) and 1 − I_DA/I_D and averaged

N	25 mM	50 mM	100 mM	200 mM	400 mM	800 mM	1 M	2 M
10	0.84 ± 0.05	0.84 ± 0.07	0.86 ± 0.04	0.87 ± 0.03	0.87 ± 0.04	0.88 ± 0.04	–	0.90 ± 0.02
15	0.66 ± 0.06	0.68 ± 0.07	0.68 ± 0.08	0.74 ± 0.05	0.76 ± 0.05	0.80 ± 0.02	–	0.84 ± 0.04
17	0.59 ± 0.002	0.60 ± 0.06	0.62 ± 0.05	0.67 ± 0.04	0.68 ± 0.07	0.74 ± 0.04	0.74 ± 0.04	0.81 ± 0.02
23	0.38 ± 0.06	0.45 ± 0.05	0.50 ± 0.08	0.54 ± 0.07	0.61 ± 0.04	0.66 ± 0.04	0.68 ± 0.04	0.72 ± 0.02
27	0.29 ± 0.05	0.35 ± 0.07	0.38 ± 0.06	0.42 ± 0.04	0.49 ± 0.06	0.55 ± 0.05	0.58 ± 0.05	0.65 ± 0.03
40	0.10 ± 0.03	0.12 ± 0.04	0.15 ± 0.03	0.21 ± 0.07	0.256 ± 0.02	0.39 ± 0.04	0.41 ± 0.03	–
70	–	0.07	0.082	0.10	0.15	0.18	0.19	0.21

The error associated with each measurement is calculated by the spread in these two measurements.