Shear stress increased EC migration speed over a range of fibronectin concentrations. Subconfluent ECs were plated on glass slides coated with fibronectin at 0.5, 1, 5, 20, and 40 μg/cm2 and then subjected to shear stress at 12 dyn/cm2 or kept as static controls. The nondirectional EC migration speed (VEC) was determined as described. Data are shown as mean ± SE. Each condition had at least 60 cells from three independent experiments. At each fibronectin concentration, the p-value was <0.05 for the difference in VEC between static and shear conditions. Under static and shear conditions, the p-values were <0.05 between VEC at 5 μg/cm2 and at other fibronectin concentration.