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. Author manuscript; available in PMC: 2026 Apr 2.
Published in final edited form as: Acta Biomater. 2024 Nov 8;191:276–291. doi: 10.1016/j.actbio.2024.11.007

Fig. 3. RNAseq evaluates global changes in gene expression related to ZNF865.

Fig. 3.

(A) Targeted upregulation of ZNF865 results in thousands of significantly differentially expressed genes. (B) The top GO Biological Processes affected by ZNF865 in ACAN/Col2a1 hASCs result in increased expression of genes related to Translation, RNA Processing, Ribosome Biogenesis, Mitochondrial Gene Expression, Gene Expression, mRNA Splicing, Mitochondrial Translation, and mRNA Processes. (C) Representative list of genes that are significantly differentially expressed genes that are associated with each term. (D) Representative transcript per million (TPM) values for ACAN, Col2a1, Col10a1, Runx2, and MMP13 in ACAN/Col2a1-ZNF865 and ACAN/Col2a1-NTC hASCs, displaying that the genes associated with a healthy chondrogenic phenotype (ACAN and Col2a1) is maintained after ZNF865 upregulation, while genes associated with chondrogenic hypertrophy (Col10a1, Runx2, MMP13) are not upregulated. NTC is nontarget control.