. 2004 May;86(5):2929–2941. doi: 10.1016/S0006-3495(04)74344-1

TABLE 1.

Spectroscopic parameters obtained from the deconvolution into three bands, N^*, H–N^*, and T^*, of the spectra of probe F in phospholipid vesicles

Sample^*	λ_N^* nm	λ_H–N^* nm	λ_T^* nm	P_N^*	P_H–N^*	P_T^*	FWHM_T^* cm⁻¹	I_N^*	I_H–N^*	I_T^*
EYPC	475	528	574	0.896	0.892	0.805	1500	0.262	0.529	0.686
DOPC	473	527	573	0.901	0.939	0.776	1500	0.283	0.777	0.563
DOPC (1/25)	473	527	574	0.900	0.905	0.765	1500	0.240	0.786	0.560
DMPC, 14°C	474	521	574	0.883	0.891	0.843	1580	0.107	0.312	0.830
DMPC, 38°C	476	527	577	0.899	0.909	0.819	1470	0.171	0.451	0.744
EYPC-Chol	471	523	574	0.882	0.894	0.841	1630	0.195	0.246	0.870
DPPC	474	521	574	0.911	0.912	0.826	1590	0.103	0.275	0.851
DLPC	475	529	575	0.905	0.900	0.783	1530	0.177	0.422	0.749
EYPG	475	527	570	0.862	0.912	0.766	1510	0.505	0.650	0.571
DOPG	481	531	571	0.826	0.900	0.809	1380	0.562	0.728	0.349
BBPS	477	527	570	0.886	0.854	0.765	1460	0.605	0.575	0.598
EYPA	479	525	569	0.900	0.900	0.770	1550	0.641	0.398	0.716

λ_N^*, λ_H–N^*, and λ_T^*, positions of the maximum of the N^*, H–N^*, and T^* bands, respectively; P_N^*, P_H–N^*, and P_T^*, asymmetries of the corresponding bands; FWHM_T^*, full width at half-maximum of the T^* band; and I_N^*, I_H–N^*, and I_T^*, peak intensities of the corresponding bands, obtained from the normalized emission spectra.

LUVs were studied at 20°C with [Probe]/[Lipid] = 1/100, unless indicated; DOPC (1/25) corresponds to [Probe]/[Lipid] = 1/25; EYPC-Chol corresponds to 30% (mol wt) of cholesterol in EYPC.