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(a) Overlap of domains is observed when the first layer contains no dye and oxidized 2:2:1 DOPC/BSM/Chol. The second layer contains the same composition, with dye. Almond-shaped domains characteristic of the first layer are observed even when there is no overlap of domains. (b–d) Supported bilayers as in (a) except the second layer contains dye and either (b) unoxidized 1:1:1 DOPC/BSM/Chol, (c) unoxidized 70:30 DOPC/Chol, or (d) unoxidized DOPC. The scale bar is 50 μm.
