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Immunofluorescence staining of C. trachomatis (L2) EBs attached to cells from the cell lines CHO-K1 and CHO-6. EBs were added to monolayers, incubated for 1 h at 4°C, fixed with methanol, and stained with a C. trachomatis-specific monoclonal antibody conjugated with fluorescein isothiocyanate.
