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. 2004 May;86(5):3042–3051. doi: 10.1016/S0006-3495(04)74353-2

FIGURE 3.

FIGURE 3

Variations in the frequency or number of pulses modify the shape of the slow calcium signal. The following protocols of electrical stimulation were used. (A) Different frequencies: the cells were exposed to 400 pulses of 1 ms at 10 Hz (squares), 45Hz (circles), or 90 Hz (triangles). (B) Different number of pulses: myotubes were exposed to 100 (squares), 400 (circles), or 600 (triangles) pulses of 1 ms at 45 Hz. All experiments were performed in the absence of extracellular calcium (0.5 mM EGTA added). The mean of at least 12 independent experiments ± SE is shown. The lower trace shows the duration of the stimulus protocol.