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Twist energy parameter, ET, versus residual linking difference of the duplex region. The solid or shaded symbols apply to 
values that are determined from the 
and 
of the simulated molecules according to Eq. 19. These 
values apply for n = 1 (shaded circles) or n = 2 (▾) melted subunits. The open symbols represent the same 
values that appear in Fig. 8 for n = 0 (□), n = 1 (○), or n = 2 (▿) melted subunits out of 155 total subunits.
