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Flash-photolysis data of selected wavelengths for the wild-type (A), R72AppR (B), R72KppR (C), R72QppR (D), and R72SppR (E). All samples were reconstituted with egg-PC. Absorbance changes in 390, 500, and 560 nm monitor the concentration of M-intermediate, ppR, and O-intermediate, respectively. The medium was 400 mM NaCl buffered with 10 mM of MES plus CHES at pH 8.0. Temperature was 20°C.
