FIGURE 6.

Effect of preelectroporation incubation time on electroporation-mediated uptake as a function of radial position of cells within multicellular spheroids. Duration of spheroid incubation in calcein solution was 10 (□), 600 (▪), and 1800 s (♦). One exponential-decay pulse of 0.48 kV/cm and 38 ms was used. The points represent experimental data from spheroids. The lines represent predictions from the model developed in this study that accounts for changes in cell state (Eq. 1), local solute concentration (Eqs. 2–6), and local electric field (Eqs. 7–9) within spheroids (see text). Average is mean ± SE.