TABLE 1.

Comparison of test parameters between the nanometer- and the micrometer-scale modulus measurements of soft biological materials by IT AFM

	Nanoregime	Microregime
Radius of the tip	Several nm (i.e., ∼20 nm)	Few μm (i.e., ∼2.5 μm)
Applied load	NanoNewton (i.e., ∼1.8 nN)	MicroNewton (i.e., 15 nN–3.3 μN)
Indentation depth	Several 10 to a few 100 nm (i.e., 300–550 nm)	Several 100 nm (i.e., 250–600 nm)