TABLE 1.

Genetic complementation of a lipolytic defect in the L. pneumophila plaA mutant

Supernatant sample	FFA (mM) released froma:
	MPLPC	MPLPG	1-MPG	DPPG	DPPC
130b(pMMB207)	0.437 ± 0.005	0.516 ± 0.018	0.169 ± 0.028	0.482 ± 0.011	0.201 ± 0.004
130b(pAF8)	3.055 ± 0.112†b	3.004 ± 0.138†	0.468 ± 0.027†	0.755 ± 0.138†	0.323 ± 0.013†
NU270(pMMB207)	0.020 ± 0.001	0.100 ± 0.011	0.081 ± 0.021	0.402 ± 0.011	0.161 ± 0.003
NU270(pAF8)	2.493 ± 0.062†	2.849 ± 0.216†	0.435 ± 0.029†	0.654 ± 0.009†	0.294 ± 0.005†

^aSupernatants from late-log phase BYE cultures of strains 130b and NU270 containing pMMB207 or pAF8 were incubated with the indicated substrates for 2.5 h at 37°C, and subsequently the release of FFA was quantified. Data are expressed as differences between the amount of FFA released by the culture supernatant and the amount released by uninoculated BYE broth. The results represent the means ± standard deviations of triplicate cultures and are representative of two independent experiments.

^bA † denotes significant differences in lipolytic activity between the wild type or plaA mutant harboring the vector pMMB207 and the respective strains with the plaA-containing vector pAF8 (P < 0.05; Student's t test).