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. 2004 Jun;86(6):3473–3495. doi: 10.1529/biophysj.103.026765

FIGURE 7.

FIGURE 7

Nuclear matrix extraction. (A) Before the nuclear matrix extraction procedure, cell nuclei appear bright with GFP-GR fluorescence. (B) In control cells almost all fluorescence disappears after nuclear matrix extraction. (C) In cells treated with sodium azide the nuclei retain a large amount of fluorescence after nuclear matrix extraction, indicating that GFP-GR is immobilized at the matrix under these conditions. Bar = 10 μm. (D) The amount of fluorescence lost after nuclear matrix extraction was quantified. In control cells 5% of fluorescence is retained (n = 99), whereas in azide-treated cells 40% of fluorescence is retained (n = 86). The large amount of fluorescence retained at the nuclear matrix in azide-treated cells matches the two-state full model prediction that 44% of GFP-GR in the nucleus is bound in a second reaction state.