TABLE 1.

Summary of model fits to large and small bleach spot FRAPs in control cells

	Large spot size (w = 1.1 μm)	Small spot size (w = 0.5 μm)
Model fit	Σ Residuals	Σ Residuals
RD	1.78	3.80
ED	0.75	2.41
FM	0.73	1.91
Two-state FM	0.73	1.90

The FRAP of GFP-GR with spot size 1.1 μm is well fit by the effective diffusion model; no additional improvement is gained with the full model. However, when the bleach spot size is reduced to 0.5 μm, the full model significantly reduces the sum of residuals, indicating an improved fit. In neither case does the two-state full model provide significant improvement.