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Immunofluorescent localization of the CTA1-CVIM constructs. CHO cells expressing either (A) CTA1-CVIM or (B) CTA1-Nglyc-CVIM were fixed and permeabilized with acetone at 48 h posttransfection, exposed to a monoclonal anti-CTA antibody for 1 h, and incubated with a FITC-conjugated goat anti-mouse IgG antibody for 30 min.
