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. 2004 Jun;86(6):3981–3992. doi: 10.1529/biophysj.103.037887

FIGURE 6.

FIGURE 6

Comparison between FCA and PCH. (a) Data of a fluorescent dye solution are analyzed by PCH and FCA. The best-fit parameters for the brightness and the number of molecules are shown together with their 68% confidence interval (FCA analysis, solid lines; PCH analysis, dotted lines). (b) Data of a binary-dye mixture of rhodamine and coumarin analyzed by PCH and FCA. The best-fit parameters for the brightness and the number of molecules of each species are shown together with their 68% confidence interval (FCA analysis, solid lines; PCH analysis, dotted lines).