FIGURE 3.

Gold-DOPE observed at a 110-μs resolution exhibited hop diffusion. What appears to be simple Brownian diffusion at a 33-ms resolution (a, video rate) is actually fast hop diffusion, as visible in recordings at a 110-μs resolution (b, 300-fold faster than the video rate). (a) Each color represents 60 step periods (every 2 s). (b) Each color indicates plausible confinement within a compartment, and black indicates intercompartmental hops. The residency time for each compartment is indicated. These compartments were detected by computer software we developed (Fujiwara et al., 2002) as well as by eye.