FIGURE 8.

Apparent D_micro (in a time-window of 100 μs based on 25-μs resolution observations) plotted against compartment size, as determined for each trajectory in the control and bleb membranes. With a larger compartment size, the apparent D_micro increased, suggesting that in smaller compartments, even a 25-μs resolution is insufficient to obtain true diffusion rates. This figure is generated using the same data set used to generate Fig. 4 (at a 25-μs resolution, solid bars) and the bleb data obtained at a 25-μs resolution. The average compartment size for NRK cells is indicated (dashed blue line). The dashed red line is a visual aid.