TABLE 3.

D_100ms, D_3s, hop rate, and residency time for Cy3-DOPE and Gold-DOPE evaluated in the membrane of FRSK cells

	D100ms (μm2/s)*		D3s (μm2/s)*
Probe	Median	Mean ± SD	Median	Mean ± SD	Hop rate† (s−1)	Residency time (ms)†	n‡
Cy3-DOPE	0.19	0.27 ± 0.20	0.15	0.18 ± 0.19	440	2.3	29 (13)
Gold-DOPE	0.044	0.086 ± 0.12	0.028	0.055 ± 0.076	100	9.8	47 (37)

For D_100ms estimates, trajectories of 0.94–16 s long (typically ∼4 s) for cy3-DOPE and 1.5–14 s long (typically ∼10 s) for Gold-DOPE were collected, and their MSD-t plots were linearly fitted between the second and the fourth points (the same as D_2–4 in Kusumi et al., 1993). For D_3s evaluations, trajectories longer than 3 s were collected, and their MSD-t plots were linearly fitted between 66 ms and 3 s.

^*D_100ms and D_3s were observed at a 33-ms resolution (video rate). The SDs include, in addition to the experimental error, the true variations in the diffusion coefficient for individual particles. The distributions for D_100ms are shown in Fig. 2.

^†The hop rates for Gold-DOPE and Cy3-DOPE were calculated using D_100ms (median), obtained by SPT of Gold-DOPE and SFVI of Cy3-DOPE (as shown in this table) and the median compartment size determined by SPT (25-μs resolution) of Gold-DOPE (41 nm), based on the relationship: τ = L²/4D_100ms ([0.041]²/4/0.19).

^‡The numbers of observed particles for D_100ms. Those for D_3s are indicated in parentheses.