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. 2004 Jun;86(6):4094–4109. doi: 10.1529/biophysj.103.036962

FIGURE 2.

FIGURE 2

Electrochromic absorption transients at a wavelength of 522 nm in F1-depleted chromatophores as caused by a short flash of light. The upper traces were obtained without and with blocking of F0 by 3 μM oligomycin, respectively. The difference trace (± oligomycin) represents the charge flow across F0 (A) without and (B) with 3 μM myxothiazol added to deactivate the electrogenic and proton pumping activity of the cytochrome bc1 complex. The actinic flash is indicated by an arrow. The bottom traces were obtained in the presence of 1 μM valinomycin to enhance the ion conductance of all chromatophores in the sample. It was apparent that absorption transients not originating from electrochromism as well as any electrochromic responses to localized electric fields were negligible under our conditions. The suspending medium contained: 100 mM KCl, 5 mM magnesium acetate, 2 mM K4[Fe(CN)6], 5 μM 1,1′-dimethylferrocene (DMF), 2 mM KCN, 20 mM glycylglycine-KOH, pH 7.9; chromatophores were added to 12 μM bacteriochlorophyll.