TABLE 1.
Bacteria, phage, and plasmids used
| Bacterium, phage or plasmid | Genotype or relevant characteristics | Derivation | Source or reference |
|---|---|---|---|
| S. enterica serovar Typhimurium strains | |||
| W | Clinical isolate S. enterica serovar Typhimurium Keller | 29 | |
| M | W mntH (Cmr) | W(pTnmntH) | This study |
| S | W sitAD (Smr) | W(pKOsitADSm) | This study |
| F | W feoB (Tetr) | W(pEP185.2-feoB::Tet) | This study |
| T | W tonB (Kmr) | P22 (AIR36) × W | This study |
| MS | S mntH (Smr Cmr) | P22 (M) × S | This study |
| MF | F mntH (Tetr Cmr) | P22 (M) × F | This study |
| SF | F sitAD (Tetr Smr) | F(pKOsitADSm) | This study |
| MT | T mntH (Kmr Cmr) | T(pTnmntH) | This study |
| MSF | SF mntH (Smr Tetr Cmr) | P22 (M) × SF | This study |
| AIR36 | IR715 tonB (Kmr) | 30 | |
| E. coli S17λpir | λ phage lysogen which contains π gene and allows replication of plasmids with an R6K origin | ||
| Phage and plasmids | |||
| P22 | P22HTint | 30 | |
| pTnMod-RCm | Plasposon with an RP4 origin for conjugation, Cmr | 8 | |
| pTnmntH | pTnModCm derivative without Tn5 transposase gene carrying an internal PCR fragment of mntH ORF (Cmr) | This study | |
| pKO3 | pSC101 derivative, which contains a temperature-sensitive origin of replication | 19 | |
| pKOsitADSm | pKO3 derivative carrying the sitAD deletion construct composed of the 5′ and 3′ ends of the operon ligated to SmR K7, replacing the central part of the operon, Cmr Smr | This study | |
| pEP185.2-feoB::Tet | pEP185.2 with a cassette conferring tetracycline resistance inserted into an internal site of a 895-bp fragment of feoB gene, Tetr | 30 | |
| pSKmntH | pBluescript SK+ carrying the complete mntH ORF, Ampr | This study | |
| pSKmntHΔ | pSKmntH derivative in which the mntH ORF contains an internal deletion between two of the SacII sites, Ampr | This study |