FIG. 2.

WC1⁺ γδ T-cell proliferation responses of four M. bovis-infected animals to PKMBSE and defined protein and nonprotein mycobacterial antigens in animals 01 (a), 02 (b), 03 (c), and 04 (b). MACS-purified WC1⁺ γδ T cells were cultured in the presence of autologous mitomycin C-treated PBMC as a source of APC. The results for individual animals are expressed as the cpm or the OD₄₅₀ (plus the standard error of the mean), as described in Fig. 1. Significant differences between control PBS-treated and antigen-stimulated cells are indicated as follows: ✽✽✽, P <0.001; ✽✽, P <0.01; ✽, P <0.05.