TABLE 1.
Data collection and refinement statistics
| Crystal | RcpA | RcpB |
|---|---|---|
| Space group | P21212 | P41212 |
| Cell dimensions (a, b, c (Å)) | 44.1, 76.7, 85.2 | 72.2, 72.2, 142.8 |
| Resolution limit (Å) | 25.0-1.90 | 20.0-1.75 |
| Wavelength (Å) | 1.050 | 1.009 |
| Measured reflections (total/unique) | 301367/22731 | 260719/39024 |
| Completeness (overall; last shell; %) | 96.9/94.6 | 98.2/98.8 |
| Rmerge* (overall; last shell; %) | 3.7/30.2 | 3.9/12.2 |
| I/σI (overall; last shell; %) | 25.2/3.1 | 27.4/12.2 |
| B-factor (Wilson/overall mean, (Å2))† | 26.4/38.2 | 22.2/26.0 |
| Final R-factor/Rfree (%) | 23.3/27.7 | 19.9/24.3 |
| RMS deviation from ideal geometry | 0.005 | 0.005 |
| Bond (Å) | 1.3 | 1.3 |
| Angles (deg) | 22.7 | 22.9 |
| Dihedrals (deg) |
*
The resolution range in the highest bin was 1.78–1.75 Å for native RcpB and 1.93–1.90 for RcpA.
†
The B-factor was estimated from a Wilson plot; for the native datasets over the resolution range 3.9–1.75 Å (RcpB) and 3.22–1.90 Å (RcpA).