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(Left) λA37G in 1 M glucose (thicker curve). The melting point in increased by 2°C compared to aqueous solution (thin curve). (Right) λD14A thermal denaturation in 0 (thin curve), 1 (medium curve), and 2 M (thick curve) glucose solutions. The transitions increases Tm, but starts earlier at higher glucose concentration. Native baselines are shown in red. A similar but even more dramatic effect is observed with ethylene glycol (not shown).
