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Mutants used in this study, and their maximum activated relaxation rates
| Name | Mutations to the protein | Maximum 1/ka and T |
|---|---|---|
| λD14A | D14A Y22W Q33Y G46A G48A | 9 μs (79°C) |
| λQ33Y | Y22W Q33Y G46A G48A | 20 μs (61°C) |
| λA37G | Y22W Q33Y A37G | 42 μs (56°C) |
| λS45A | Y22W Q33Y G46A S45A G48A | 25 μs (56°C) |
| λS79A | Y22W Q33Y G46A S45A G48A S79A | 24 μs (59°C) |
| λA49G | D14A Y22W Q33Y A37G G46A G48A A49G | 29 μs (54°C) |
