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. 2004 Jul;87(1):675–687. doi: 10.1529/biophysj.103.032714

FIGURE 3.

FIGURE 3

50.3-MHz 13C{19F} REDOR dephasing (ΔS/So) for a 50-50 mixture of [1-13C]Ala10-[15N]Gly11-(KIAGKIA)3-NH2 and [2-2H, 3-19F]Ala10-(KIAGKIA)3-NH2, incorporated into synthetic MLVs of DPPG/DPPC (1:1) at a lipid/peptide molar ratio of 20 (○) and 40 (▴). Root mean-square deviation errors are indicated by the vertical lines for the first six evolution times (L/P = 20). Both samples were lyophilized in a protecting sugar matrix. The crosses show dephasing for the frozen fully hydrated (L/P = 20) sample of Fig. 2 (top). The solid lines show the calculated dephasing assuming two distributions of 13C-19F pair distances. At L/P = 20, the two distributions are about equally populated and the difference in average dephasing rates for the two populations is more than a factor of two. Based on signal/noise ratios, the root mean-square deviation uncertainty in the dephasing (ΔS/So) is estimated at 5% (see footnote to Table 1). One distribution has a mean internuclear 13C-19F separation of 4.5 Å with a width of 0.7 Å, and the other distribution has a mean of 9.6 Å and a width of 3.0 Å. At L/P = 40, the population with the smaller mean and narrower distribution width decreases.