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. 2004 Jul;87(1):675–687. doi: 10.1529/biophysj.103.032714

FIGURE 5.

FIGURE 5

50.3-MHz 15N → 13C{19F} TEDOR-REDOR NMR spectra of a 50-50 mixture of ([3-13C]Ala3-[15N]Gly4-[1-13C]Ala10-[2-13C]Gly11-[6-15N]Lys12-[1-13C]Ala17-[15N]Gly18-(KIAGKIA)3-NH2 and [2-2H, 3-19F]Ala14-(KIAGKIA)3-NH2), incorporated into synthetic MLVs of DPPG/DPPC (1:1) at a lipid/peptide molar ratio of 20 after 128 rotor cycles of dipolar evolution with magic-angle spinning at 5000 Hz. The spectrum at the bottom resulted from a 4-Tr, 15N→13C TEDOR coherence transfer followed by 128 additional rotor cycles with high power proton decoupling. The 128-Tr REDOR ΔS spectrum of the TEDOR-selected signal is shown at the top of the figure.