FIGURE 1.
Single-channel features of hCt in the absence and in the presence of SDS with associated histograms of the conductance fluctuations. (a) hCt 125 nM; (b) hCt 125 nM + SDS (0.26 mM); (c) hCt 24.5 nM; (d) hCt 24.5 nM + SDS (0.26 mM); (e) hCt 5 nM; and (f) hCt 5 nM + SDS (5 pM). Experiments were performed on a POPC/DOPG (85:15) membrane in the presence of hCt and of hCt + SDS added to the cis-side; the voltage was set to +150 mV, the aqueous phase contained 1M KCl (pH 7), and T = 22°C. Note the increase in channel occurrence (channels/minute) when SDS was present in the medium.
