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Parameters derived from the fluorescence anisotropy of acrylodan-I27 complexes
| Sample | pH | τ1 (ns) | g1 | τ2 (ns) | g2 | r0 | χ2 |
|---|---|---|---|---|---|---|---|
| I27NHT | 7.0 | 5.85 ± 0.61 | 0.266 ± 0.009 | 0.87 ± 0.02 | 0.074 ± 0.002 | 0.340 | 0.5 |
| I27SHT | 7.0 | 6.65 ± 0.20 | 0.246 ± 0.005 | 1.16 ± 0.05 | 0.097 ± 0.008 | 0.343 | 0.3 |
| I27SHT | 4.5 | 6.85 ± 0.20 | 0.258 ± 0.008 | 1.11 ± 0.10 | 0.087 ± 0.008 | 0.345 | 0.4 |
