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. 2004 Aug;87(2):1360–1368. doi: 10.1529/biophysj.103.037895

FIGURE 1.

FIGURE 1

Single-photon excitation spectra of SBFI in buffer solutions (A) and in rat ventricular myocytes (B) at various [Na]. Spectra were recorded on the stage of an epifluorescence microscope, at λem = 535 ± 20 nm, and were not corrected for the transmission characteristics of the optical components. To obtain the spectra of SBFI when loaded into myocytes, at different [Na]i, the cells were perfused with divalent-free solutions with various [Na]o in the presence of 10 μM gramicidin D and 100 μM strophanthidin (see Materials and Methods).