FIGURE 5.

Time-resolved fluorescence intensity decay of the channel conformation of gramicidin in POPC vesicles. Excitation wavelength was at 297 nm which corresponds to a peak in the spectral output of the nitrogen lamp. Emission was monitored at 340 nm. The sharp peak on the left is the lamp profile. The relatively broad peak on the right is the decay profile, fitted to a biexponential function. The two lower plots show the weighted residuals and the autocorrelation function of the weighted residuals. All other conditions are as in Fig. 1. See Materials and Methods for other details.