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. 2004 Aug;87(2):858–872. doi: 10.1529/biophysj.103.037671

FIGURE 1.

FIGURE 1

SKOR assembles with plant Kout but not with plant Kin channel α-subunits after coexpression in Xenopus oocytes. (A) Representative inward and outward K+ currents obtained from oocytes injected with a SKOR/KAT1 cRNA mixture (1:1 ratio). From a holding potential of −100 mV currents were evoked by 3.5-s activating voltage pulses from −160 mV to +70 mV (10-mV steps). (B) Steady-state current-voltage characteristic (solid line) for the oocyte shown in A, superimposed with the respective current-voltage relationship for homomeric KAT1 (shaded circles) and SKOR (open triangles). (C and D) Steady-state K+ current amplitudes after coexpression (1/1 cRNA mixtures) of SKOR (C) and GORK (D) with dominant-negative mutants of SKOR (dnSKOR = SKOR-G292R-D293N-A296V) and GORK (dnGORK = GORK-G273R-Y274R). Current amplitudes were measured at the end of 3-s voltage pulses to +80 mV. To compare results obtained from different oocyte batches, the data were normalized to the mean value (Iss) of the respective controls SKOR/H2O and GORK/H2O. Data are shown as means ± SD (n = 6–17, two different oocyte batches).