FIGURE 7.

Identification of weak intermolecular interactions between Ct-SKOR and different C-terminus constructs in the yeast two-hybrid system. (A) Generation of chimeric/truncation mutants on the basis of the SKOR (i) and the KAT1 (v) C-terminus. The individual C-termini are represented schematically (i–vi). Parts originating from KAT1 are illustrated by white rectangles, and parts from SKOR are shown as black rectangles. The label “I” indicates the proximal interacting region of SKOR, the label “I′” its counterpart found in KAT1, and the label “σ” the distal interacting region. Note that in construct (ii) the proximal interacting region was partly deleted. The constructs were fused to the GAL4 activator domain. (B) Identification of protein interactions between Ct-SKOR (fused to the LexA binding domain) and the constructs shown in A by analyzing β-galactosidase activities. Data are shown as means ± SD (n = 4–8). To facilitate the comparison all values were normalized to the mean value measured for the positive control (self-interaction of Ct-SKOR = 100%, i in panel A). The dotted line indicates the background level of β-galactosidase activity measured with Ct-SKOR in combination with the empty pAD-GAL4 vector (vi).