TABLE 1.
Nucleotide and magnesium composition of electrode solutions
| *[ADP]free (mM) | †[ATP]total (mM) | *[MgATP] (mM) | †[ADP]total (mM) | *[Mg]free (mM) | [Mg]total (mM) |
|---|---|---|---|---|---|
| 0 | 15.0 | 13.2 | 0 | 1.0 | 15.0 |
| 0.4 | 14.3 | 12.3 | 0.7 | 0.9 | 14.3 |
| 0.8 | 13.4 | 11.8 | 1.6 | 1.1 | 14.5 |
| 1.7 | 11.0 | 10.0 | 4.0 | 1.3 | 14.5 |
| 3.0 | 7.0 | 6.5 | 8.0 | 1.7 | 14.5 |
| 4.3 | 4.0 | 3.7 | 11.0 | 1.6 | 13.2 |
[EGTA] = 10 mM was included in the calculations.
The MgATP complex was arbitrarily chosen as the enzyme catalytic substrate.
For experimental evidence supporting the choice of free ADP as the relevant variable in this work, see online supplementary material.
*
Calculated with the program MaxChelator (supplied by Chris Patton, Hopkins Marine Station, Pacific Grove, CA) using the set of constants “CMC1002S.TCM”, pH = 7.4, T = 23°C, ionic strength = 0.2.
†
[ATP]total + [ADP]total = 15 mM.