FIGURE 5.
Negative gating polarity of T8R channels. (A) Cell-attached records of a single T8R record at the indicated holding potentials. The open probability of the channel increases as the holding potential becomes more positive. (B) A plot of the Popen/voltage relation of T8R. The number of records used to obtain the mean and standard deviation are in the parentheses. Data were fitted to a sigmoidal function using Origin 6.0 software. Records were obtained with pipette and bath solutions composed of 140 mM KCl, 1 mM EDTA, and 10 mM HEPES, pH 7.6.